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Ultrasonic Courtship Vocalizations of Adult Male Mice: A Laboratory Exercise Illustrating Comparable Activation by either Estradiol or Testosterone

This article describes a laboratory exercise that links hormonal regulation to reproductive behaviors in adult male mice. It focuses on the regulatory function of the hormone, E2 and the relationship to ultrasonic courtship vocalizations in response to a female or the presence of her odor. Discussion questions and modifications are included to further analyze the link between hormones and reproductive behavior.

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