HomeAbout

SIGN IN   Advanced Search










 
Browse Review
Fanciful FRET

Fo¨rster resonance energy transfer (FRET) is used to measure protein-protein interactions in living cells. When FRET occurs, a "donor" fluorophore is excited by the absorption of a photon, and that energy is transferred by a nonradiative mechanism to a nearby acceptor molecule. The fraction of energy-transfer events relative to donor excitation events is called the FRET efficiency. It has a steep dependence on the separation distance between the donor and acceptor. Proteins of interest tagged with spectral variants of green fluorescent protein (GFP) can be readily used for FRET studies in living cells. The proper interpretation of FRET measurements, however, requires an understanding of the limitations of the numerous methods used for measuring FRET, as well as consideration of the subtleties of FRET theory, particularly its dependence on the abundance of donors and acceptors. This review highlights aspects of the acquisition of FRET measurements, and of FRET theory, that are vital for proper interpretation. The adoption of "standards" with known FRET efficiencies is a first step toward eliminating erroneous interpretations of FRET experiments due to errors in the accuracy and precision of FRET measurements.

Rate this Resource:
1 = not useful, 5 = very useful

Please be the first to rate this resource.


Subscribe and
View Resource

Classifications


Resource Type: Bibliography, Diagram, Illustration, Journal article/Issue, Review
Audience Level: Undergraduate upper division 15-16, Graduate, Professional (degree program)

Author and Copyright


Authors and Editors: Steven S. Vogel of National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Christopher Thaler of National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Srinagesh V. Koushik of National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health
Publisher: American Association for the Advancement of Science
Format: application/pdf, image/gif, image/jpeg, text/html
Copyright and other restrictions: Yes
Cost: Yes

Comments


» Sign In or register to post comments.


Collection:
STKE/Science Signaling


     
   

SITE MAP | CONTACT | POLICIES

Triple A S National Science Foundation Naitonal Science Digital Library Pathway
Funded by the individual BEN Collaborators and grants from the
National Science Foundation [DUE 0085840 / DUE 0226185 / DUE 0532797 / DUE 0734995]

This website is a National Science Digital Library (NSDL) Pathway.
Copyright © 2018. American Association for the Advancement of Science. All Rights Reserved.