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Optical Sectioning and Confocal Microscopy

Confocal microscopy is a powerful technique for acquiring three-dimensional images of biological samples. Here I discuss the basic principles of confocal microscopy, with specific discussions of the operation of laser scanning and spinning disk confocal microscopes and of their application to biology.

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Classifications


Resource Type: Video, Lecture/Lecture outline, Laboratory exercise
Audience Level: High school upper division 11-12, Undergraduate lower division 13-14, Undergraduate upper division 15-16

Author and Copyright


Authors and Editors: Kurt Thorn of Nikon Imaging Center
Publisher: iBioSeminars
Format: text/html
Copyright and other restrictions: Yes
Cost: No

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