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Sticky Caveats in an Otherwise Glowing Report: Oligomerizing Fluorescent Proteins and Their Use in Cell Biology

Fluorescent proteins from sea creatures have revolutionized the study of cell biology and signal transduction in many ways. Zacharias discusses some of the technical caveats to working with these proteins when they are fused to cellular proteins to track protein localization and interactions. Special attention is paid to problems arising from oligomerization of these fluorescent proteins and how that impacts protein interactions detected by fluorescence resonance energy transfer (FRET).

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Classifications


Resource Type: Illustration, Journal article/Issue, Review
Audience Level: Undergraduate upper division 15-16, Graduate, Professional (degree program)

Author and Copyright


Authors and Editors: David A. Zacharias of Merck Research Laboratories
Publisher: American Association for the Advancement of Science
Format: application/pdf, image/gif, image/jpeg, text/html
Copyright and other restrictions: Yes
Cost: Yes

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Collection:
STKE/Science Signaling


     
   

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