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DNA Barcoding

This is a two-part animation. “DNA Barcoding, Part 1,” provides an overview of how DNA barcoding of animals can be used to identify an unknown sample or discover a new species. Cytochrome c oxidase subunit 1 (COI) is found in the mitochondria as part of the electron transport chain. The COI gene is used for DNA barcoding. Just like a barcode on an item in a grocery store identifies a product, a DNA barcode (determined by DNA sequencing) is used to identify species. Part 1 run time: 1 minute, 40 seconds. “DNA Barcoding, Part 2” details how small tissue samples are used for DNA barcoding, including a review of the laboratory and bioinformatics steps used in barcoding: DNA purification, polymerase chain reaction (PCR), agarose gel electrophoresis, DNA sequencing and analysis, and DNA sequence identification using the Basic Local Alignment Search Tool (BLAST) or the Barcode of Life Database (BOLD). Part 2 run time: 4 minutes, 15 seconds. Animation is closed captioned.

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Resource Type: Animation, Tutorial
Audience Level: High School lower division (Grades 9-10), High School upper division (Grades 11-12), Undergraduate lower division (Grades 13-14), Undergraduate upper division (Grades 15-16), Informal Education

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Northwest Association for Biomedical Research



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