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Light Microscopy Techniques for Live Cell Imaging

This article discusses the primary approaches used in the study of dynamic cellular events. Since the earliest examination of cellular structures, biologists have been fascinated by observing cells using light microscopy. The advent of fluorescent labeling technologies plus the plethora of sophisticated light microscope techniques now available make studying dynamic processes in living cells almost commonplace. For anyone new to this area, however, it can be daunting to decide which techniques or equipment to try. Here, we aim to give a brief overview of the main approaches to live cell imaging, with some mention of their pros and cons.

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Resource Type: Journal article/Issue
Audience Level: High school upper division 11-12, Undergraduate lower division 13-14, Undergraduate upper division 15-16, Graduate, Professional (degree program), Continuing education

Author and Copyright


Authors and Editors: David Stephens of Department of Biochemistry, University of Bristol, Victoria Allan of School of Biological Sciences, School of Medical Sciences
Publisher: SCIENCE
Format: text/html
Copyright and other restrictions: Yes
Cost: No

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