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Development and Use of Fluorescent Protein Markers in Living Cells

This article discusses advances in fluorescent proteins and fluorescent imaging techniques. The ability to visualize, track, and quantify molecules and events in living cells with high spatial and temporal resolution is essential for understanding biological systems. Only recently has it become feasible to carry out these tasks due to the advent of fluorescent protein technology. Here, we trace the development of highly visible and minimally perturbing fluorescent proteins that, together with updated fluorescent imaging techniques, are providing unprecedented insights into the movement of proteins and their interactions with cellular components in living cells.

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Classifications


Resource Type: Journal article/Issue, Illustration
Audience Level: High school upper division 11-12, Undergraduate lower division 13-14, Undergraduate upper division 15-16, Graduate, Professional (degree program), Continuing education

Author and Copyright


Authors and Editors: Jennifer Lippincott-Schwartz of Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, George Patterson of Cell Biology and Metabolism Branch, National Institutes of Health
Publisher: SCIENCE
Format: text/html
Copyright and other restrictions: Yes
Cost: No

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American Association for the Advancement of Science


     
   

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