HomeAbout

SIGN IN   Advanced Search










 
Browse Biotechnology
Quantitative Analysis of Protein-Lipid Interactions Using Tryptophan Fluorescence

The fluorescent properties of the amino acid tryptophan make it a useful tool for fluorometric assays. Because tryptophan fluorescence is remarkably sensitive to the polarity of the environment, it can be used to determine the affinity of tryptophan-containing peptides for phospholipid vesicles of varying compositions. Here, we describe a method for using tryptophan fluorescence to determine the binding affinities of peptides derived from the proteins Raf-1 and KSR-1 to small unilamellar vesicles containing phosphatidic acid. The method can be extrapolated to measure the binding of other tryptophan-containing peptides or proteins to lipid vesicles.

Rate this Resource:
1 = not useful, 5 = very useful

Please be the first to rate this resource.


Subscribe and
View Resource

Classifications


Resource Type: Diagram, Illustration, Image, Journal article/Issue, Laboratory manual, Table
Audience Level: Undergraduate upper division 15-16, Graduate, Professional (degree program)

Author and Copyright


Authors and Editors: Catherine A. Kraft of The Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Jose Luis Garrido of The Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Luis Leiva-Vega of The Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Guillermo Romero of The Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine
Publisher: American Association for the Advancement of Science
Format: application/pdf, image/gif, image/jpeg, text/html
Copyright and other restrictions: Yes
Cost: Yes

Comments


» Sign In or register to post comments.


Collection:
STKE/Science Signaling


     
   

SITE MAP | CONTACT | POLICIES

Triple A S National Science Foundation Naitonal Science Digital Library Pathway
Funded by the individual BEN Collaborators and grants from the
National Science Foundation [DUE 0085840 / DUE 0226185 / DUE 0532797 / DUE 0734995]

This website is a National Science Digital Library (NSDL) Pathway.
Copyright © 2019. American Association for the Advancement of Science. All Rights Reserved.