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Protein Purification Supplemental: Buffers and Assays

This resource addresses the following Core Concepts of Vision and Change: 1. EVOLUTION 2. STRUCTURE AND FUNCTION 4. PATHWAYS AND TRANSFORMATIONS OF ENERGY AND MATTER This resource can help students develop the following Core Competencies of Vision and Change: 1. ABILITY TO APPLY THE PROCESS OF SCIENCE 2. ABILITY TO USE QUANTITATIVE REASONING 5. ABILITY TO COMMUNICATE AND COLLABORATE WITH OTHER DISCIPLINES These materials are intended to be part of a Vision and Change Collection about enzyme purification. This lab activity involves preparing biological buffers, determining and adjusting pH, and performing spectrophotometric assays to measure enzyme activity and determine protein concentration. These are tested materials currently used in a seven week advanced laboratory course that is targeted at juniors and seniors (BIOC 311: Advanced Experimental Biosciences). Although the course web site was created in 1999 and has undergone frequent revisions, I just introduced the “lecture chunks” (by breaking up my traditional 60 minute Ppt lectures into 3-5 minute clips) and SCALE-UP format during the fall 2011 semester. I also have several DEMO videos that were filmed by my students that were introduced in the spring 2012 semester. I have additional Ppt slides that I use during the class discussion before lab that complement the student-centered activities.

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Resource Type: Digital Presentation (Powerpoint), Short Movies, Table, Assessment: other, Laboratory or Hands-On Activity, Laboratory Manual, Lecture / Lecture Outline / Lecture Notes, Teaching Strategies & Guidelines, Tutorial
Audience Level: High School lower division (Grades 9-10), High School upper division (Grades 11-12), Undergraduate lower division (Grades 13-14), Undergraduate upper division (Grades 15-16)

Author and Copyright

Authors and Editors: PhD Beth Beason-Abmayr of Rice University Biochemistry and Cell Biology
Publisher: Rice University Biochemistry and Cell Biology
Copyright and other restrictions: No
Cost: No


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