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Protein Purification 1: Extraction and Precipitation

This resource addresses the following Core Concepts of Vision and Change: 1. EVOLUTION 2. STRUCTURE AND FUNCTION 4. PATHWAYS AND TRANSFORMATIONS OF ENERGY AND MATTER This resource can help students develop the following Core Competencies of Vision and Change: 1. ABILITY TO APPLY THE PROCESS OF SCIENCE 2. ABILITY TO USE QUANTITATIVE REASONING 5. ABILITY TO COMMUNICATE AND COLLABORATE WITH OTHER DISCIPLINES These materials are intended to be part of a Vision and Change Collection about enzyme purification. This lab activity begins the partial purification of an enzyme from native and recombinant sources. The initial step in purification involves the extraction of soluble proteins from the source: the cells are lysed or the tissue is homogenized, and cell debris is removed by centrifugation. As a second purification step, proteins are precipitated using ammonium sulfate. Students work in teams (typically of 3-4) to complete the objectives and analyze the data. The pre-lab lecture component uses SCALE-UP to engage the students and prepare them for lab. Students watch “lecture chunks” (created by breaking up my traditional 60 minute Ppt lectures into 3-5 minute video clips) before the pre-lab meeting: these short presentations about biochemical theory and equipment set-up allow the instructor to “flip the classroom” (i.e., learn before lecture) so class time can incorporate more student-centered activities. I have additional Ppt slides with clicker questions that I use during the class discussion before lab that complement the student-centered activities. These are tested materials currently used in a seven day advanced undergraduate laboratory course (BIOC 311: Advanced Experimental Biosciences) that is targeted at juniors and seniors.

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Classifications


Resource Type: Digital Presentation (Powerpoint), Short Movies, Assessment: other, Laboratory or Hands-On Activity, Laboratory Manual, Lecture / Lecture Outline / Lecture Notes, Teaching Strategies & Guidelines, Tutorial
Audience Level: High School lower division (Grades 9-10), High School upper division (Grades 11-12), Undergraduate lower division (Grades 13-14), Undergraduate upper division (Grades 15-16)

Author and Copyright


Authors and Editors: PhD Beth Beason-Abmayr of Rice University Biochemistry and Cell Biology
Publisher: Rice University Biochemistry and Cell Biology
Copyright and other restrictions: No
Cost: No

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Collection:
American Physiological Society


     
   

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