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Introduction to Enzyme Kinetics: Assay of beta-Galactosidase

This tutorial describes a colorometric enzyme assay to determine simple enzyme kinetics. It is designed as an introduction for beginning laboratory students in a biology or biochemistry course, and requires no previous understanding of chemistry. It may be used as an assignment to prepare students for a laboratory exercise in enzyme kinetics Through this tutorial, students will gain an understanding of the procedures for measuring enzyme activity, using the specific example of an assay for the bacterial enzyme, β-galactosidase.

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Resource Type: Assignment/Activity (non-laboratory), Illustration, Lecture/Lecture outline
Audience Level: High school upper division 11-12, Undergraduate lower division 13-14, Undergraduate upper division 15-16

Author and Copyright

Authors and Editors: Joanne Kivela Tillotson of Purchase College, State University of New York
Publisher: American Association for the Advancement of Science
Format: application/pdf, application/x-shockwave-flash, application/x-zip-compressed, image/gif, image/jpeg, text/html
Copyright and other restrictions: Yes
Cost: No


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